INVESTIGADORES
PEICHOTO Maria elisa
artículos
Título:
Biochemical and biological analysis of Philodryas baroni (Baron's Green Racer; Dipsadidae) venom: relevance of the findings to human risk assessment
Autor/es:
MATIAS NICOLAS SANCHEZ; ALEJANDRO TIMONIUK; SILVANA MARU√ĎAK; PAMELA TEIBLER; OFELIA ACOSTA; MARIA ELISA PEICHOTO
Revista:
HUMAN EXP. TOXICOL.
Editorial:
SAGE PUBLICATIONS LTD
Referencias:
Lugar: London; Año: 2014 vol. 33 p. 22 - 31
ISSN:
0960-3271
Resumen:
Philodryas baroni - an attractively colored snake - has become readily available through the exotic pet trade. Most people consider this species harmless; however, it has already caused human envenomation. As little is known about the venom from this South-American opisthoglyphous ?colubrid? snake, herein, we studied its protein composition by SDS-PAGE, as well as its effects on the hemostatic system. Both reducing and non-reducing SDS-PAGE analysis demonstrated that the venom exhibits greatest complexity in the range of 50-80 kDa. The venom displayed proteolytic activity towards azocollagen, with a specific activity of 75.5 U/mg, and rapidly hydrolyzed the Aα-chain of fibrinogen, exhibiting lower activity towards the B- and γ-chains. Philodryas baroni venom showed no platelet pro-aggregating activity per se, but it inhibited collagen- and thrombin-induced platelet aggregation. Prominent hemorrhage developed in mouse skin after intradermal injection of the crude venom, and its minimum hemorrhagic dose was 13.9 μg. When injected intramuscularly into the gastrocnemius of mice, the venom induced local effects such as hemorrhage, myonecrosis, edema and leucocyte infiltration. Due to its venom toxicity shown herein, P. baroni should be considered dangerous to humans and any medically significant bite should be promptly reviewed by a qualified health professional.