INVESTIGADORES
SANSBERRO pedro Alfonso
artículos
Título:
Direct shoot regeneration from leaf and internode explants of Aloysia polystachya [Gris] Mold. (Verbenaceae)
Autor/es:
BURDYN, L.; LUNA, C.; TARRAGÓ, J; SANSBERRO, P.; DUDIT, N.; GONZÁLEZ, A.; MROGINSKI, L.
Revista:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. PLANT
Editorial:
Society for In Vitro Biology
Referencias:
Lugar: USA; Año: 2006 vol. 42 p. 235 - 239
ISSN:
1054-5476
Resumen:
Adventitious bud regeneration from leaf and internode explants of Aloysia polystachya was achieved. Shoots from nodal segments grown in vitro were cut into pieces and used as source of explants. Organogenesis was induced from both explants cultured on ¼ strength Murashige and Skoog (plus sucrose 5 gr·L-1) semisolid medium containing different combinations of 6-benziladenine (BA) and a-naphtalenacetic acid (NAA) under 116 mmol·m-2·s-1 PPFD (photoperiod 14 h) and 27±2ºC. The type of explants markedly influenced organogenesis and the growth of the regenerated shoots. The regeneration frequencies were higher with leaf explants while the number of shoots formed per responsive explant was greater with internodes. However, the growth of regenerated shoots from internodes was seriously affected by vitrification. The number of shoots produced per responsive leaf explant increased from 1 to 7, as the percentage of leaf explants producing shoots increased from 20 to more than 80%. NAA 0.05 mM in combination with BA 0.5mM induced the highest regeneration rate (87±8.8%) after 20 days of culture bringing 5.9±0.8 shoots per responsive leaf explant. Histological examination confirmed the direct process of organogenesis. The regenerated shoots from the best induction treatment were transferred to a fresh medium without plant growth regulators for 30 days. Finally, the elongated shoots were rooted by pre-treatment in an aqueous solution of NAA 500 mM for 2 h and transferred to ¼ MS. All plantlets raised in vitro were phenotypically normal and successfully hardened to ex vitro conditions. An experimental field plots with 2-year-old in vitro regenerated plants were established.