In vitro culture of zygotic embryos of Ilex species.

SANSBERRO, P.; REY, HY; MROGINSKI, LA

HortScience

American Society for Horticultural Science

Año: 2001 vol. 36 p. 351 - 352

0018-5345

Plants of Ilex argentina L., I. brasiliensis (S.) L., I. brevicuspis R., I. dumosa R., I. integerrima (V.C.) L., I. microdonta R., I. pseudoboxus R., and I. theezans C.M. were obtained by immature embryo culture. Heart-stage zygotic embryos were removed from immature fruits and cultured aseptically on quarter-strength Murashige and Skoog medium containing 3% sucrose, 0.65% agar and 0.1 mg.L-1 zeatin. Cultures were incubated at 27 ± 2°C, for 4 weeks, in darkness and subsequently transferred to a culture room with 14 h photoperiod (116 mmol. m-2.s-1) for another 4 weeks. Seedlings derived from the germinated embryos, with two leaves were successfully transplanted to pots containing peat, perlite and sand (1:1:1 v/v) and were maintained in greenhouse conditions. A range of 95 - 100% of survival rates after transplanting was obtained