INVESTIGADORES
SANSBERRO pedro Alfonso
artículos
Título:
In vitro plantlets regeneration of Schinopsis balansae (Anacardiaceae).
Autor/es:
PEDRO ALFONSO SANSBERRO; REY, HY; MROGINSKI, L. A.; LUNA, C. V.
Revista:
TREES-STRUCTURE AND FUNCTION
Editorial:
Springer-Verlag
Referencias:
Lugar: Heidelberg; Año: 2003 vol. 17 p. 542 - 546
ISSN:
0931-1890
Resumen:
A protocol for the in vitro regeneration of rooted plants from nodal single bud segments of 10-year-old Schinopsis balansae trees was developed. Nodal segments were harvested from actively growing shoots of plants grown from seeds and maintained in pots in greenhouse conditions, and from epicormic shoots obtained by forced flushing of branches. Culture of nodal segments on nutrient medium containing the mineral salts and vitamins of Murashige and Skoog medium at 1/4 strength (1/4MS), supplemented with 100 mg l-1 ascorbic acid, 3% sucrose, and 5–15 mM 6-benzyladenine (BA) resulted in regeneration of multiple shoots. Rooting of regenerated shoots was observed in 1/4 MS medium with vermiculite as the substrate and supplemented with 7.5 mM indolbutyric acid (IBA).