Plant regeneration from Ilex spp. (Aquifoliaceae)in vitro

PEDRO ALFONSO SANSBERRO; REY, HY; KRIVENKI, MA; MROGINSKI, LA

BIOCELL

Instituto de Histología y Embriología "Dr. Mario H. Burgos"

Lugar: Mendoza; Año: 2001 vol. 25 p. 139 - 146

0327-9545

In vitro plant regeneration from nodal segments (containing one axillary buds) of seven species of the genus Ilex (I. argentina, I. brevicuspis, I. dumosa, I. microdonta, I. pseudoboxus, I. taubertiana, I. theezans) were readily acchieved through three steps: 1 shoot regeneration by in vitro culture of nodal segments in MS medium at ¼ strength, plus 3% sucrose and 0.65% agar (¼ MS) and 0.5 µM BA (45 days of culture); 2) Induction of rooting from regenerated shoot with ¼ MS with 7.3 µM IBA (7 days) and, 3) subculture of shoots in a fresh medium (1/4 MS lacking plant growth regulators) during 21 days. Shoot regeneration of other three species (I. aquifolium, I. brasiliensis, and I. integerrima) were also obtained by in vitro culture of nodal segments. Shoot regeneraqtion of I. aquifolium, I. brasiliensis,  I. integerrima, I. microdonta, I. pseudoboxus,and I. taubertiana were also obtained by culture of shoot tips on ¼ MS and 0.5 µM BA. Shoot regeneration from meristems of I. argentina, I. brevicuspis, I. dumosa, and I. theezans were readily achieved by in vitro culture on the same medium.