INVESTIGADORES
SANSBERRO pedro Alfonso
artículos
Título:
Influence of gelling agents on Ilex paraguariensis tissue culture.
Autor/es:
PEDRO ALFONSO SANSBERRO; REY, HY; LUNA, C.; MROGINSKI, LA
Revista:
ACTA HORTICULTURAE
Editorial:
International Society for Horticultural Science.
Referencias:
Lugar: USA; Año: 2001 vol. 560 p. 453 - 456
ISSN:
0567-7572
Resumen:
The effect of gelling agents on different in vitro tissue culture systems of Ilex paraguariensis was studied. Interaction were found between type of agar and plant regeneration. The type of agar, being Phytagel significantly better than agar (A-1296) affected somatic embryogenesis by in vitro culture of immature embryos. However, agargel promote induction of callus from leaves, while Phytagel was the best gelling agent for callus growth. Althouhg, the type of gelling agent did not affect the in vitro stablishment of nodal segments, the multiplication phase as well as the rooting of regenerated shoot was greatly increased by Phytagel. Phytagel was also found to be more adequate gelling agent for in vitro maturation and germination of immature zygotic embryos.