Micropropagation of Ilex dumosa (Aquifoliaceae) from nodal segments in a tissue culture system

LUNA, C. V.; PEDRO ALFONSO SANSBERRO; MROGINSKI, L. A.; TARRAGÓ, J. R.

Biocell

Instituto de Histología y Embriología "Dr. Mario H. Burgos"

Lugar: Mendoza; Año: 2003 vol. 27 p. 205 - 212

Micropropagation of Ilex dumosa var. dumosa R. ( “yerba señorita”) from nodal segments containing one axillary bud was investigated. Shoot regeneration from explants of six-year-old plants was readily achieved in ¼ strength Murashige and Skoog medium (¼MS) plus 30 gr·L-1 sucrose and supplemented with 4.4 mM BA. Further multiplication and elongation of the regenerated shoots were obtained by subculture in a fresh medium of similar composition with 1.5 gr·L-1 sucrose. Rooting induction from shoots were achieved in two steps: 1) 7 days in ¼ MS (30 gr·L-1 sucrose, 0.25 % PhytagelÒ) with 7.3 mM IBA and 2) 21 days in the same medium without IBA and 20 mM of cadaverine added. Regenerated plants were successfully transferred to soil. This micropropagation schedule can be implemented in breeding programs of Ilex dumosa.