SANSBERRO pedro Alfonso
Uso de distintos desinfectantes superficiales para el establecimiento in vitro de segmentos nodales de Grevillea robusta
BOGADO F.; VERA BRAVO C.; AYALA, P.; SANSBERRO, P.; LUNA, C.; LUNA, C.
Universidad nacional de Rosario
Lugar: Rosario; Año: 2016 vol. 27 p. 11 - 16
Microbial contamination is a constant problem, which often compromises development of all in vitro techniques. The losses caused by contaminating microorganisms, mainly fungi and bacteria, are a serious worldwide problem in numerous laboratories. Thereby, to determine a protocol for the disinfection of nodal segments of Grevillea robusta from sprouts collected from greenhouse plants, different disinfectant solutions were tried (sodium hypochlorite; potassium hydrogen persulfate; hydrogen peroxide; and two fungicides, copperoxychloride and carbendazim), using different concentrations(between 0.5 and 10 g. L-1) and times of exposure (15, 30, and 60 minutes). The explants were cultivated in a basal medium of Murasghige and Skoog. At 28 days of culture, the percentages of contamination, oxidation and survival/establishment of all theexplants were determined. Even though all treatments tested allowed establishment between 36.67± 5.77 and 90.00±10 %,the best results were obtained with a pretreatment using NaClO 0.25 g. L-1 and Carbendazim 0.75 g. L-1 for 15 minutes. Thus,this pre-treatment is recommended for a successful establishment, with a minimum percentage of contamination,and phytotoxic effects of blackening or tissue oxidation withinreasonable parameters.