INVESTIGADORES
LUCCHESI paula Maria Alejandra
artículos
Título:
Multiplex PCR assay for the detection of five putative virulence genes encoded in verotoxigenic Escherichia coli plasmids
Autor/es:
BUSTAMANTE, A.V.; SANSO, A. M.; LUCCHESI, P.M.A.; PARMA, A. E.
Revista:
CURRENT MICROBIOLOGY
Editorial:
SPRINGER
Referencias:
Año: 2011 vol. 62 p. 1411 - 1415
ISSN:
0343-8651
Resumen:
The aim was to perform a pentavalent PCR
assay for the detection of putative virulence genes encoded
in VTEC plasmids, katP, espP, subA, stcE, and ehxA. The
five-specific primer pairs used in the assay do not interfere
with each other and generate amplification products of 914,
774, 556, 399, and 262 bp. It was selected at random 39
strains belonged to 20 serotypes in order to evaluate the
multiplex in a wide variety of strains. The results of this
study indicate that it is possible to perform simultaneous
amplification and search for recognized plasmid-encoded
virulence markers from different E. coli serotypes and
apply this technique to the genetic characterization of
E. coli strains isolated from reservoirs, foods or patients.
This complementary technique is a useful tool to detect
interstrain differences for epidemiological studies and to
provide information that could be related to the risk of
human infection.
