INVESTIGADORES
LUCCHESI paula Maria Alejandra
artículos
Título:
Detection of an Antigenic Protein of Leptospira interrogans
Autor/es:
PARMA, A. E.; SANZ, M.E.; LUCCHESI, P.M.A.; MAZZONELLI, J.; PETRUCCELLI, M. A.
Revista:
The Veterinary Journal
Editorial:
ELSEVIER SCI LTD
Referencias:
Año: 1997 vol. 153 p. 75 - 79
ISSN:
1090-0233
Resumen:
A protein epitope which is involved in an antigenic relationship between equine ocular tissues and Leptospira interrogans was detected in homogenates of the bacterium. The antigenic determinant was harboured on a peptide structure which was shown to be sensitive to the action of denaturing and reducing agents by means of Western blotting. The outer surface of the leptospires appeared to be free of this epitope as was proved by dot-blot and electron microscopic studies.was detected in homogenates of the bacterium. The antigenic determinant was harboured on a peptide structure which was shown to be sensitive to the action of denaturing and reducing agents by means of Western blotting. The outer surface of the leptospires appeared to be free of this epitope as was proved by dot-blot and electron microscopic studies.