Isolation and Purification Of mccP136 a Cold Active Bacteriocin Produced By a Psychrophilic Serratia sp

SÁNCHEZ L A; DELGADO O D

Buenos Aires, Argentina

Congreso; 43° Reunión Anual SAIB; 2007

SAIB

Antimicrobial peptides are molecules widespread in life forms to mediate competition, and their industrial production could be important for the potential use as preservative in food, cosmetical and pharmaceutical industries. Cold environments could be a suitable source of microorganisms with ability to produce cold-active antimicrobials of biotechnological interest. Objectives: The aim of the present study was to lab-scale produce and purify MccP136, a widespread-pathogen inhibition spectrum molecule by using a psychrotrophic Serratia sp.Methods: Batch fermentations were carried out in a BIOFLO III bioreactor under pH, temperature and DO controlled conditions. The growth curve and MccP136 production were determined during the fermentation process. The MccP136 was collected during the process in the foam phase and centrifuged, cell-free supernatant was subjected to a two step purification procedure: Solid Phase extraction in a C18 RP-Cartridge followed by HPLC. Results: The MccP136 was produced on 5L batch culture at 8ºC and the cell-free supernatant was used in order to develop an efficient purification procedure.Conclusions: We have evaluated and developed a cost-efficient production and purification process for MccP136, an antimicrobial compound produced at low temperatures with a broad pH range of activity against a broad spectrum of widely known pathogenic bacteria.