INVESTIGADORES
DELGADO osvaldo Daniel
artículos
Título:
Pseudomonas yamanorum sp. nov., a pshychrotolerant moderate halophile bacterium isolated from Isla de los Estados, Ushuaia Argentina.
Autor/es:
ARNAU VG; SANCHEZ LA; DELGADO OD
Revista:
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY.
Editorial:
SOC GENERAL MICROBIOLOGY
Referencias:
Lugar: London; Año: 2015 vol. 65 p. 424 - 431
ISSN:
1466-5026
Resumen:
A psychrotolerant strain, 8H1T, was isolated from soil samples collected in Isla de los Estados, Ushuaia, Argentina. Cells were Gram-negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by means of two polar flagella. The isolate was able to grow in the range 4?35 6C, with optimum growth at 28 6C. The predominant cellular fatty acids were summed feature 3 (C16 : 1v6c and/or C16 : 1v7c), C16 : 0 and summed feature 8 (C18 : 1v6c and/or C18 : 1v7c). The polar lipid pattern of strain 8H1T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. The DNA G+C content was 59.8 mol%. 16S rRNA gene sequence-based phylogeny suggested the affiliation of strain 8H1T to the ?Pseudomonas fluorescens group?, displaying ¢98.5% sequence similarity to 29 type strains. A multilocus sequence analysis (MLSA) study performed by concatenating 16S rRNA, gyrB, rpoD and rpoB gene sequences showed that isolate 8H1T could be discriminated from closely related species of the genus Pseudomonas and placed in the ?Pseudomonas gessardii subgroup?, including the species with the highest MLSA sequence similarities: Pseudomonas brenneri (96.2 %), P. gessardii (96.1 %), P. proteolytica (96.0 %), P. meridiana (96.0 %) and P. mucidolens (95.4 %). DNA?DNA hybridization analysis between 8H1T and the type strains of these closely related species revealed relatedness values of 27.0, 8.8, 41.2, 39.7 and 46.1 %, respectively. These results, together with differences in several phenotypic features, support the classification of anovel species, for which the name Pseudomonas yamanorum sp. nov. is proposed. The typestrain is 8H1T (5DSM 26522T5CCUG 63249T5LMG 27247T).