Chromosomal integration of green fluorescent protein in Zymomonas mobilis.

DELGADO O D; MARTINEZ M A; ABATE C M; SIÑERIZ F

BIOTECHNOLOGY LETTERS

Springer

Lugar: Londres; Año: 2002 vol. 24 p. 1285 - 1290

0141-5492

An integrative vector was constructed to allow expression of heterologous proteins into the adhB locus of Zymomonas mobilis. As a reporter gene, the ORF of a bright variant of green fluorescent protein from Aequorea victoria (GFPuv) was fused to the adhB strong promoter from Z. mobilis by using a two-step PCR strategy. Z. mobilis recombinant strains that were stably marked by precise gene replacement at adhB locus with a single chromosomal copy of gfpuv. Protein expression was confirmed by fluorescence microscopy and measured by fluorescence spectroscopy, showing high expression levels (12 to 30 times higher than those obtained in E. coli) without affecting the host growth.