INVESTIGADORES
ORDOÑEZ Maria victoria
congresos y reuniones científicas
Título:
Identification and Characterization of Ubiquitin-like Proteins in Haloarchaea
Autor/es:
ORDOÑEZ MV; NERCESSIAN D; CONDE RD
Lugar:
Pinamar, Pcia Buenos Aires, Argentina
Reunión:
Congreso; SAIB 41th Annual Meeting, SAN 20th Annual Meeting and PABMB 10th Congress; 2005
Institución organizadora:
Sociedad Argentina de Investigaciones en Bioquimica y Biología Molecular
Resumen:
Ubiquitin is a small protein, whose structure is very conserved among eucaryotes. It is involved in the mechanism of protein degradation. There is not genomic evidence supporting the presence of ubiquitin in prokaryotes. However, although their sequences are different, certain archaeal proteins show structural and functional homology with ubiquitin. In this work we report the finding of proteins that react with an anti-ubiquitin antibody in different genera of halophilic archaea as analyzed by Western blot. Like ubiquitin, some of these proteins resulted heat-stable and reacted with the antibody after heating for 1 hour at 86°C. For a further characterization, the cell protein extracts were subjected to 2D SDS-PAGE. The subsequent immunoblot assay of the gels revealed many spots. These proteins are actually being further purified for sequencing. The obtained sequences will allow to establish whether they have either sequential or conformational homology, or both, with ubiquitin and related proteins. Supported by UNMdP, CONICET and Fundación Antorchas.