IANNONE Leopoldo Javier
congresos y reuniones científicas
Foliar grass endophytes increase diversity and colonization of arbuscular mycorrhizal fungi
ARRIETA, A; IANNONE, L.J; SORIA, M.; VIGNALE, M.V; MC CARGO, P.D.; NOVAS, M.V.
Simposio; 32nd. New Phytologist Symposium; 2013
New Phytologist Trust
Asexual Epichloë endophytes establish mutualistic symbioses with different species of grasses affecting the interaction between their hosts and other microorganisms. Here we analyze the effect of Epichloë pampeana, one endophyte of Bromus auleticus, on arbuscular mycorrhizal fungi (AMF) diversity through classical and metagenomics approaches. Endophyte-infected seeds (E+) or noninfected (E-) were sown in pots filled with agricultural (A) or non-agricultural (NA) soil collected at the Pampean regions. One year later, roots were evaluated for mycorrhizal colonization and DNA was extracted from soil, spores and roots to assess mycorrhizal diversity by high-throughput 454- pyrosequencing of the 18S rRNA gene. Mycorrhizal colonization was higher in E+ plants. Metagenomics analyses evidenced a higher number of reads in soil of E- plants but diversity of AMF was higher in roots of E+ plants. Our results show that asexual Epichloë endophytes promote the establishment of AMF and increase its diversity, supporting the hypothesis of a positive effect of asexual Epichloë on mycorrhizal colonization of native grasses.