INVESTIGADORES
MORATA vilma ines
artículos
Título:
High-throughput modified QuEChERS method for the determination of the mycotoxin tenuazonic acid in wine grapes
Autor/es:
FONTANA, ARIEL; PRENDES, L. P.; MORATA DE AMBROSINI V.I.; BOTTINI, RUB√ČN AMBROSIO
Revista:
RSC Advances
Editorial:
ROYAL SOCIETY OF CHEMISTRY
Referencias:
Lugar: Sheffield; Año: 2016 vol. 6 p. 95670 - 95679
ISSN:
2046-2069
Resumen:
A reliable, simple, fast, inexpensive and robust sample preparation approach for tenuazonic acid (TA)determination in grapes by liquid chromatography with ultraviolet detection (HPLC-UV) is proposed. The method is based on a modified QuEChERS (quick, easy, cheap, effective, rugged and safe) approachusing ethyl acetate as extraction solvent. Its involves extraction of 2.5 g sample (plus 2.5 mL 1% formic acid acidified water) with 5 mL of ethyl acetate; drying/cleaning-up of extract with 0.25 g anhydrous CaCl2 and determination by HPLC-UV. The method was optimized and validated achieving a limit ofquantification (LOQ) of 0.05 mg g 1. The overall recoveries were 96%, 82% and 97% for grape samples spiked at 0.05, 0.5 and 5 mg g 1, respectively. The method showed excellent reproducibility with RSDs for the above data #8% and Horwitz ratios