INVESTIGADORES
MONTANER Alejandro Daniel
congresos y reuniones científicas
Título:
Effects of a continuous and prolonged IMT504 treatment in diabetic female NOD mice
Autor/es:
BIANCHI, STEFANIA; LAVIGNOLLE HEGUY, MARÍA DEL ROSARIO; MONTANER, ALEJANDRO; LIBERTUN, CARLOS; LUX-LANTOS, VICTORIA A.; BIANCHI, MARÍA S.
Lugar:
Buenos Aires
Reunión:
Jornada; XX Jornadas anuales de la Sociedad Argentina de Biología y XVII Jornada de la Sociedad Uruguaya de Biociencias; 2018
Institución organizadora:
Sociedad Argentina de Biología
Resumen:
We have shown that the immunomodulatory oligonucleotide IMT504 (IMT) promotes marked recovery of toxic diabetes in rats that correlates with early expression of progenitor cell markers but without altering immune parameters. IMT also improves immunodependent diabetesin mice, diminishing glycemia and reducing leukocyte islet infiltration. Besides, a short-term IMT treatment induces an early recovery of glucose homeostasis and insulitis in NOD mice,a model of type I diabetes. Here, we evaluated the effects of a continuous chronic IMT treatmentin NOD diabetic mice, more similar to what could eventually be used inhumans.Diabetic female NOD mice (non-fasted glycemia (Gly) levels between 250-350 mg/dl were s.c. implanted with constant drug release pumps (Alzet osmotic pumps) with a capacity for 28 days, loaded with IMT (total dose/ per day: 20 mg/kg body weight (BW) or saline (diabetic control: DC) (day1). Gly and BW were determined once a week during the treatment. The weekly food intake was determined. On day 21, glucose tolerance (ipGTT) and insulin secretion (IST) testswere performed. On day 28, fasted mice were sacrificed, blood samples and pancreases collected for hormonal determinations and histological studies respectively. We observed that 22% of NOD mice showed spontaneous reversion of the diabetic condition whereas IMT treatment ameliorated Gly in 78% ofmice (X2: p