Effects of oligodeoxynucleotide IMT504 on cell viability and gene expression in a murine beta cell line (min6b1)

CONVERTI A; A MONTANER; LIBERTUN C; LUX LANTOS V; BONAVENTURA MM

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedades de Biociencias de Argentina

Bioengineering strategies will allow the development of new approaches for the treatment of bone loss. Biocompatible matrices containing bio-modulating molecules, involved in cell signaling and in cell-extracellular matrix interaction, are a promising alternative to treat critical bone injuries. The aim of this study was to evaluate a bone substitute, constituted by a collagen matrix embedded with BMP-2 or oligonucleotide (ODN) 504, in the healing of critical bone lesions in a rat calvaria model. Bone substitutes were generated using Hellitape (collagen type I membrane) containing BMP-2 500ng/ml or Biopad (collagen type I sponge) containing oligonucleotide (ODN) 504 1mg/ml. These constructs and their specific controls (matrices) were applied to surgery-generated critical calvaria lesions (10 male Wistar rats per group). An absolute control group (untreated lesion) was also performed. Half of the animals in each group were sacrificed after 10 days and another half after 40 days post-surgery. The analysis of the remaining bone lesion area was performed using Image-J software from X-rays images. Ten days post-surgery, radiographic studies did not show significant differences in wound closure areas between groups. Even if 40 days post-surgery, all groups showed a significant decrease in the lesion area compared to 10 days post-surgery, only the Hellitape-BMP2 group and its control showed a significantly improved closure of the lesion. In particular, the Hellitape-BMP2 group showed the greatest closure when compared to its control. Our results support the Hellitape-BMP2 construct as a promising bone substitute to treat critical bone lesions. However, a greater number of animals should be analyzed. Keywords: Bioengineered bone substitutes, BMP-2, Oligonucleotides, bone injury healing.