INVESTIGADORES
MONTANER Alejandro Daniel
congresos y reuniones científicas
Título:
Oligonucleotide IMT504 improves hyperglycemia, polyphagia, polydipsia and islet morphology in streptozotocin-induced diabetes in rats
Autor/es:
MS BIANCHI; A HERNANDO-INSÚA; JM RODRÍGUEZ; F ELÍAS; C MIRABELLI; N LAGO; J ZORZOPULOS; NA CHASSEING; C LIBERTUN; A MONTANER; V LUX-LANTOS
Lugar:
San Francisco, CA (USA)
Reunión:
Congreso; The Endocrine Society. 90th Annual Meeting; 2008
Institución organizadora:
The Endocrine Society (USA)
Resumen:
  IMT504, the prototype of the PyNTTTTGT class of immunomodulatory oligonucleotides (ODN), stimulates Mesenchymal Stem Cells (MSC) expansion both in vitro and in vivo (1). We evaluated the effect of IMT504 administration on blood glucose, solid and liquid food-intake, and pancreatic islet morphology in streptozotocin (STZ)-induced diabetes in rats.   Male Sprague Dawley rats (200-250 g BW) were injected with STZ i.p. (60 mg/kg, diluted in citrate buffer) or citrate buffer as control (C). Animals with blood glucose levels 200 mg/dl (range: 200-500 md/dl) 3 days after STZ were considered diabetic and injected subcutaneously with daily doses of ODN IMT504 (4 mg/dose) for 5 days, 2 days of rest, and another 5 days (10 doses in all, STZ-ODN) or saline as control (STZ). Food and water intakes were recorded and glycemia was measured from tail blood with a glucometer every four days for a total of 30 days. Thereafter, glucose tolerance tests (GTT) were performed to study islet beta cell function (2g/kg BW glucose was injected ip and glucose determined in blood samples at 0, 30, 60, and 120 min). Two days later animals were sacrificed, blood samples and pancreata were collected for hormonal determinations and histological studies respectively.  Treatment with ODN effectively lowered blood glucose in 33% of STZ-ODN-treated rats (STZ-ODN+=4/12) [Day 15: Blood glucose (mg/dl): C=122 3 (n=11), STZ=477 31 (n=8), STZ-ODN+=125 17 (n=4), p