INVESTIGADORES
HAMANN monika Ines
congresos y reuniones científicas
Título:
Molecular biology techniques applied to characterize Ribeiroia spp. from northern Argentina
Autor/es:
DAVIES C.; LAUTHIERT J. J.; HAMANN M . I.; OSTROWSKI DE NÚÑEZ M.
Lugar:
Tucumán
Reunión:
Jornada; XXVIII Jornadas Científicas de la Asociación de Biología de Tucumán; 2011
Resumen:
Cercariae assigned to Ribeiroia sp for showing two esophageal diverticles were found in Biomphalaria species from Salta (B. tenagophila and B. orbignyi) and Corrientes (B. occidentalis) provinces, Argentina. Prevalence vary between 0.2 % and 0.6 %. B. occidentalis cercariae had few granular cystogenous cells, veil around the acetabulum and lacked pre-pharyngeal body; B. tenagophila cercariae lacked pre-pharyngeal body; B. orbignyi cercariae had pink pre-pharyngeal body, para-esophageal glands and cystogenous cells with bar-shaped contents. R. ondatrae cercariae from North American Helisoma tivolvis, possess acetabula with teeth, pink pre-pharyngeal tissue and few cystogenous cells. R. marini cercariae, from Caribbean B. glabrata, lacked pink tissue, had toothless, unveiled acetabula and many cystogenous cells. Cercaria lileta (R. congolensis) had pink tissue. The present work compare the genetic sequences of Argentinean cercariae to those already published for R. marini, R. ondatrae and Cercaria lileta. DNA was extracted with Qiagen kit, and PCR performed with primers ITS 2 (Wilson et al., 2005), yielding a 650 bp product. Sequences were compared to GeneBank database (megablast). We found 2.5 % divergence between Argentinean samples and those already published, suggesting different species in agreement with morphological analysis.