INVESTIGADORES
COLLAVINO monica Mariana
artículos
Título:
Identification and control of bacterial contaminants from Ilex dumosa nodal segments culture in a temporal immersion bioreactor system using 16S rDNA analysis
Autor/es:
CLAUDIA LUNA; MÓNICA COLLAVINO; PEDRO SANSBERRO; LUIS MROGINSKI
Revista:
PLANT CELL TISSUE AND ORGAN CULTURE
Editorial:
Springer
Referencias:
Año: 2008 vol. 95 p. 13 - 19
ISSN:
0167-6857
Resumen:
Endogenous bacterial contaminants isolated
from infected cultures of Ilex dumosa nodal
segments were identified as Stenotrophomonas maltophilia
and Achromobacter sp. using 16S rDNA
analysis. A range of antibiotics with different mechanism
of actions and the commercial biocide PPMTM
were tested for their capacity to repress the growth of
Gram negative bacteria grown in liquid medium
during the establishment phase of temporal immersion
systems. The best results were obtained with the
addition of 0.5 mg ml-1 cefotaxime to the culture
media obtaining 100% of uncontaminated cultures
without suppress of shoot growth.