INVESTIGADORES
COLLAVINO monica Mariana
artículos
Título:
In vitro plant regeneration of Ilex paraguariensis St. Hil. (Aquifoliaceae)
Autor/es:
SANSBERRO P. A., H.Y. REY, L.A. MROGINSKI Y M.M. COLLAVINO
Revista:
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. PLANT
Referencias:
Año: 1999 vol. 35 p. 401 - 402
ISSN:
1054-5476
Resumen:
Nodal segments as well as shoot tips and apical meristems of 2-yr-old 'mate plants (Ilex paraguariensis St. Hil.) were cultured in vitro to establish micropropagation systems. Maximum shoot regeneration was achieved when nodal segments were cultured with 1/4 Murashige and Skoog (MS) medium with 3% sucrose. We induced roots to differentiate by transferring the regenerated shoots onto the same medium, solidified with 2.5 g Phytagel per 1 and supplemented with indole-3-butyric acid (7.4 ìM) and finally transferring shoots to 1/4 MS medium with 3% sucrose and lacking growth regulators. Plants were successfully established in soil.