B. cereus phospholipase C engineering for efficient degumming of vegetable oil

CLAUDIA ELENA; SEBASTIAN CERMINATI; PABLO RAVASI; RODOLFO RASIA; SALVADOR PEIRU; HUGO G. MENZELLA; MARIA CASTELLI

PROCESS BIOCHEMISTRY - (Print)

ELSEVIER SCI LTD

Lugar: Amsterdam; Año: 2017 vol. 54 p. 57 - 62

1359-5113

Enzymatic phospholipid removal (degumming) is a fast-growing and environmentally friendly process for vegetable oil refining. Type C phospholipases (PLC) are the preferred enzymes since they provide anextra yield in the oil recovery.Bacillus cereus PLC can hydrolyze phosphatidylcholine (PC) but has a limited efficiency at removing phosphatidylethanolamine (PE), which together represent ∼70% of the phospholipids present in crude soybean oil. In the present work, we show that the B. cereus PLC mutant F66Y can remove up to 90% of PE while retaining its efficiency at hydrolyzing PC. Oil treatment with the engineered enzyme provides an extra yield of 1.84% making the B. cereus PLC F66Y mutant an attractive candidate for its industrial use.