High level production of Bacillus cereus phospholipase C in Corynebacterium glutamicum

PABLO RAVASI; MAURICIO BRAIA; FLORENCIA EBERHARDT; CLAUDIA ELENA; SEBASTIAN CERMINATI; SALVADOR PEIRU; MARIA CASTELLI; HUGO MENZELLA

JOURNAL OF BIOTECHNOLOGY

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2015 vol. 216 p. 142 - 148

0168-1656

AbstractEnzymatic oil degumming using phospholipase C enzymes is a well-established and environmentally friendly process for vegetable oil refining. In this work, we report the recombinant production of Bacillus cereus PLC in Corynebacterium glutamicum ATCC 13869 in a high cell density fermentation process and its performance in soybean oil degumming. A final concentration of 5.5 g/L of the recombinant enzyme was achieved when the gene was expressed from the tac promoter in a semi-defined medium. After treatment with trypsin to cleave the propeptide, the mature enzyme completely hydrolyzes phosphatidylcholine and phosphatidylethanolamine, which represent 70% of the phospholipids present in soybean oil. The results presented here show the feasibility of using B. cereus PLC for oil degumming and provide a scalable manufacturing process for the cost effective production of this enzyme.