Industrial uses of phospholipases: current state and future applications

CERMINATI, SEBASTIÁN; PAOLETTI, LUCIANA; AGUIRRE, ANDRÉS; PEIRÚ, SALVADOR; MENZELLA, HUGO G.; CASTELLI, MARÍA EUGENIA

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

SPRINGER

Lugar: Berlin; Año: 2019

0175-7598

AbstractPhospholipids play a central role in all living organisms. Phospholipases, the enzymes aimed at modifying phospholipids, areconsequently widespread in nature and play diverse roles, from lipid metabolism and cellular signaling in eukaryotes to virulenceand nutrient acquisition in microbes. Phospholipases catalyze the hydrolysis of one or more ester or phosphodiester bonds ofglycerophospholipids. The use of phospholipases with industrial purposes has constantly increased over the last 30 years. Thisdemand is rapidly growing given the ongoing improvements in protein engineering and the reduction of enzymes manufacturingcosts, making them suitable for industrial use. Here, a general overview of phopholipases A, B, C, and D and their industrialapplication is presented along with potential new uses for these enzymes.We draw attention to commercial phospholipases usedto improve the emulsifying properties of products in the baking, egg, and dairy industries. On the other hand, the improvement ofoil degumming by phospholipases is thoroughly analyzed. Moreover, recent developments in enzymatic biodiesel productionand the use of phospholipases for the synthesis of phospholipids with pharmaceutical or nutritional value are reviewed.