INVESTIGADORES
RISSO marikena Guadalupe
artículos
Título:
Antibody maturation in Trypanosoma cruzi-infected rats
Autor/es:
MARCIPAR IS, RISSO MG, SILBER AM, REVELLI S, MARCIPAR AJ
Revista:
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY.
Editorial:
American Society for Microbiology
Referencias:
Año: 2001 vol. 8 p. 802 - 805
ISSN:
1071-412X
Resumen:
The study of antibody avidity changes during infection has improved the understanding of the pathologic processes involved in several infectious diseases. In some infections, like toxoplasmosis, this information is being used for diagnostic purposes. Results of the evolution of antibody avidity for different specific antigens in Trypanosome cruzi-infected rats are presented. A Western blotting technique, combined with avidity analysis to identify antigens that elicit high-avidity antibodies, is suggested. In this system, antibodies showed high avidity values only during the chronic phase of infection and only in relation to antibodies against 21-, 33-, 41-, 42-, 56-, 58-, 66-, and 72-kDa antigens. Finally, a 97-kDa T. cruzi antigen, which was recognized by high-avidity antibodies and occurred in noninfected rats, was identified. These results allow us to evaluate the different antigens in chagasic infection. Our results show that with the correct choice of antigen it is possible to detect differences in maturation of antibodies and to discriminate, in an experimental model, between recent (acute) and chronic infections.