INVESTIGADORES
RISSO marikena Guadalupe
congresos y reuniones científicas
Título:
Differential expression of trans-sialidase between Trypanosoma cruzi phylogenetic lineages correlates to their contrasting biological behaviour
Autor/es:
LEGUIZAMÓN M SUSANA; RISSO MARIKENA; MOCETTI ESTEBAN; GARBARINO GLORIA; GONZÁLEZ CAPPA STELLA M; BUSCAGLIA CARLOS; CAMPETELLA OSCAR
Lugar:
Curitiba, Brasil
Reunión:
Simposio; I AMSUD Pasteur Meeting “Host-Pathogen Interactions”; 2003
Institución organizadora:
Programa AMSUD-Pasteur
Resumen:
Trypanosoma cruzi population is conformed by two major phylogenetic groups (T.cruzi-I and T.cruzi-II). T. cruzi-II is more prevalent in chagasic patients from endemic areas and is associated with Chagas’ disease pathologies. Using a mouse infection model we found that T. cruzi-II stocks induce 100 % mortality during the acute phase of the infection whereas T. cruzi I stocks are non-lethal and led to the establishment of a chronic infection. T. cruzi II bloodstream trypomastigotes express and shed significantly higher amounts of trans-sialidase in in vivo and ex vivo assays. We have previously described a role of trans-sialidase in the development of thymus alterations observed during the acute phase of the infection. Accordingly, T. cruzi-II-infected mice presented progressive thymus damage leading to the loss of normal organ histoarchitecture by the time of animal death. T. cruzi I-infected animals, showed only slight and transient thymic lesions and underwent complete organ recovery coinciding with the decline in parasitemia and the establishment of a chronic infection and the developing of trans-sialidase neutralizing antibodies. This is the first report showing the differential expression of a parasite virulence factor that might underlie the contrasting behavior of the two T. cruzi phylogenetic groups in the mammalian host.