INVESTIGADORES
MARANI mariela Mirta
congresos y reuniones científicas
Título:
Patagonian amphibians´host-defence peptides show antimicrobial, anticancer and antiparasitic properties
Autor/es:
ARRULO MIRIAM F.; AMARAL CONSTANCA P.; MARANI MARIELA M.; CANCELARICH NATALIA L.; DOMINGUES MARCO M.; SANCHES-VAZ MARGARIDA; FIGUEIREDO LUÍSA M.; SANTOS NUNO C.; EATON PETER
Lugar:
Lisboa
Reunión:
Encuentro; Chemistry: Shaping the future & 2019 summer school - 4th Meeting of the College of Chemistry of ULisboa (4ECQUL); 2019
Institución organizadora:
Universidade de Lisboa y Colegio de Química
Resumen:
Increasing antibiotic resistance, in a multitude of infectious agents, alongside with the low rate of new antibiotics discovery culminated in an urgent need to search for new alternatives. Host-defence peptides (HDPs) are usually short, cationic amphipathic molecules that form part of the innate immune system of most multicellular organisms [1]. Initially described as antimicrobial peptides (AMPs), recent research has shown that HDPs possess a wider range of actions, such as anticancer properties, thus also being designated as anticancer peptides (ACPs) [2]. The overall aim of this work was to characterise anticancer, antimicrobial and antiparasitic properties of five HDPs that originate from the skin of Patagonian amphibians: PSo-4, PSo-7, PSo2-2, Thau-3 and Ooc-1. Antimicrobial properties were assessed against Escherichia coli (ATCC® 25922?) and Staphylococcus aureus (ATCC® 25923?), by minimal inhibitory concentration (MIC) determination. The mechanism of action of the peptides was investigated via atomic force microscopy (AFM). Additionally, antiparasitic assays were performed on wild-type (wt) bloodstream forms of Trypanosoma brucei brucei Lister 427. Viability was assessed by analysing the fluorescence produced as a result of the reduction of alamarBlue by surviving parasites [3] and confirmed by optical microscopy. Anticancer activity was also evaluated, by quantifying the viability of human hepatocellular carcinoma Huh-7 cells after exposure to the peptides (XTT assay) [4]. Current results show that only peptides PSo-4 and Ooc-1 exhibited antimicrobial activity against E. coli, but merely at high concentrations, and none of the peptides tested produced any effect on S. aureus. Also, preliminary data points towards all of the peptides exerting inhibitory effects on carcinoma Huh-7 cells while T. brucei brucei Lister 427 wt parasites seem to be particularly susceptible to the action of Ooc-1. References: [1] Robert E. W. Hancock, Hans-Georg Sahl; Nature Biotechnology. 24 (2006) 1551-1557. [2] Diana Gaspar, A. Salomé Veiga, Miguel A. R. B. Castanho; Frontiers in Microbiology. 4 (2013) 294. [3] B. Räz, M. Iten, Y. Grether-Bühler, R. Kaminsky, R. Brun; Acta Tropica. 68 (1997) 139-147. [4] Özlem Sultan Aslantürk. In Vitro Cytotoxicity and Cell Viability Assays: Principles, Advantages, and Disadvantages. In: Marcelo L. Larramendy, Sonia Soloneski (Eds.), Genotoxicity - A Predictable Risk to Our Actual World, IntechOpen Ltd. London, 2018, pp. 1-18.