INVESTIGADORES
MARANI mariela Mirta
congresos y reuniones científicas
Título:
Extensive in silico evaluation of CP4-EPSPS peptides as potential biomarkers of transgenic soybean.
Autor/es:
COSTA JOANA B.; MARANI MARIELA M.; MAFRA ISABEL; OLIVEIRA MARÍA B.P.P.; CAMPERI SILVIA A.; LEITE JOSÉ R. S. A.
Lugar:
Parnaíba
Reunión:
Workshop; Biosensors & Biotechnology. Proteomics and Nanobiosensors: A new concept for GMO assessment.; 2014
Institución organizadora:
Universidad Federal do Piauí-BIOTEC
Resumen:
Nowadays, with the advances of bioinformatics, there is a diversified set of tools for the prediction of linear and conformational epitopes in proteins, mostly based on T- and B-cell prediction softwares. For the prospective immunorecognition of 5-enolpyruvulshikimate-3-phosphate synthase (CP4-EPSPS) as a biomarker protein expressed by transgenic soybean, the CP4-EPSPS protein was in silico cleaved with trypsin/chymotrypsin and the resultant peptides were extensively evaluated for further selection of the best candidate peptides for antibody production. The main objective of this study was selecting a set of peptides that could function as potential immunogens for the production of novel antibodies against the CP4-EPSPS protein. To accomplish this goal, the available bioinformatics, namely DiscoTope, ElliPro or POPI 2.0 softwares, among several other tools, were employed for the evaluation of a total of 32 sequences ranging from 10 to 25 amino acids in length. The analysis enabled the successful proposal of four peptides with potential immunogenicity for the future use as screening biomarkers of genetically modified organisms. To our knowledge, this is the first attempt to select and define potential linear epitopes for the immunization of animals and subsequently, to generate adequate antibodies for the immunorecognition of CP4-EPSPS. At the present moment, the selected peptides are already being synthesized and they are expected to be incubated in animals for antibody generation with potential applicability for the development of an immunosensor for CP4-EPSPS detection.