INVESTIGADORES
MARANI mariela Mirta
congresos y reuniones científicas
Título:
One-bead one-peptide library two-stage screening followed by MALDI-TOF MS/MS for ligand design
Autor/es:
MARTÍNEZ CERON MARÍA C.; GIUDICESSI SILVANA L.; KRUSZYN JOHANNA N.; MARANI MARIELA M.; ALBERICIO FERNANDO; CASCONE OSVALDO; CAMPERI SILVIA A.
Lugar:
Cayo Santa María
Reunión:
Simposio; Simposio Internacional de Química; 2013
Institución organizadora:
Sociedad Cubana de Química, UCLV Universidad Central "Marta Abreu" de las Villas
Resumen:
Short peptides are excellent ligands for affinity chromatography as they are more stable than antibodies to elution and cleaning conditions and they usually have very aceptable selectivity for the target protein and stability against proteases. Divide-couple-recombine (DCR) method allows obtaining a library with all posible combinations of the amino acids in the form of "one-bead one-peptide". In this work we designed a two stage library screening method for peptide ligand selection.