INVESTIGADORES
MARANI mariela Mirta
artículos
Título:
Isolation of trypsin from bovine pancreas using immobilized benzamidine and peptide CTPR ligands in expanded beds
Autor/es:
IANNUCCI NANCY B.; ALBANESI GUILLERMO J.; MARANI MARIELA M.; FERNÁNDEZ LAHORE HÉCTOR M.; CASCONE OSVALDO; CAMPERI SILVIA A.
Revista:
SEPARATION SCIENCE AND TECHNOLOGY
Editorial:
Taylor & Francis LLC
Referencias:
Año: 2005 vol. 40 p. 3277 - 3287
ISSN:
0149-6395
Resumen:
Peptide CTPR and p-amino benzamidine (PAB) immobilized on StreamlineTM were utilized as the chromatographic matrices for trypsin purification from bovine pancreas. By using a clarified pancreas extract, maximum capacity for CTPR-Streamline was 47.4 mg/mL and for PAB-Streamline 78.9 mg/mL while Kd values were 0.39 and 0.38 respectively. When the purification process was applied to unclarified pancreas extract in the expanded-bed adsorption mode, 80% trypsin recovery with a purification factor of 18.7 was achieved. Cationic and anionic trypsin obtained from the affinity column were separated by ion-exchange chromatography.