INVESTIGADORES
GONZALEZ Ana maria
congresos y reuniones científicas
Título:
Anticandidal and antioxidant activities from leaf extracts of climbers of selected species of Bignoniaceae family
Autor/es:
TORRES C; SÁNCHEZ C.; VIVIANI M.; NÚÑEZ M; GONZALEZ A.
Lugar:
Córdoba
Reunión:
Congreso; III Reunión Internacional de Ciencias Farmacéuticas,; 2014
Resumen:
Bignoniaceae is a family of shrubs, trees and climbers. It comprises tropical species and many are used as traditional medicines. The scavenging activity against DPPH (1,1-diphenil-2-picrylhydrazyl) and ABTS (2,2′-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid) radicals and the anticandidal effect of hydroetanolic extracts of leaves of seven climbers were investigated. The selected species were: Adenocalymma marginatum (Cham.) DC., Amphilophium vauthieri DC, Cuspidaria convoluta (Vell.) A. H. Gentry, Dolichandra dentata (K. Schum.) L. G. Lohmann, Fridericia caudigera (S. Moore) L.G. Lohmann, F. chica (Bonpl.) L. G. Lohmann and Tanaecium selloi (Spreng.) L. G. Lohmann. The anticandidal activity was determined using agar disc diffusion and microdilution in broth method. The antifungal activity of each extract was tested against: Candida albicans ATCC 10231, Candida parapsilosis ATCC 22019 and clinical isolates of C. albicans, C. glabrata, C. kruzei, C. parapsilosis y C. tropicalis. Zones of inhibition of extracts were compared with that of fluconazole. The results showed that the most active extracts were A. vauthieri, C. convoluta, F. caudigera y T. selloi. Their minimal inhibitory concentration ranged from 125 to 1000 µg of phenolic compounds/mL. The antioxidant activity was evaluated by the DPPH and ABTS methods. The scavenging capacity was confirmed in all of cases. The antioxidant activity was expressed as Trolox equivalent antioxidant capacity (TEAC). The TEAC results revealed that the ability to scavenge DPPH (A. marginatum ~ F. chica ~ F. caudigera > C. convolute > T. selloi > A. vauthieri > D. dentata), differed from the ability to scavenge ABTS+ (C. convolute ~ F. chica ~ D. dentata > A. marginatum > F. caudigera ~ A. vauthieri > T. selloi). Overall, C. convoluta and F. caudigera were the most promising species. Hence, these plants can be used to discover bioactive natural products that may serve in the development of new pharmaceuticals.