BUONOCORE BIANCHERI maria josefina
Eﬀects of host age and radiation dose in Diachasmimorpha longicaudata (Hymenoptera: Braconidae) mass-reared on medﬂy larvae of the tsl Vienna8 genetic sexing strain
LORENA SUAREZ; MARIA JOSEFINA BUONOCORE BIANCHERI; FERNANDO MURUA; MARIANA BILBAO; MELISA GARCIA; JORGE CANCINO; OSCAR MARTIN; DIEGO MOLINA; OSVALDO LARIA; OVRUSKI, SERGIO MARCELO
ACADEMIC PRESS INC ELSEVIER SCIENCE
Lugar: Amsterdam; Año: 2019 vol. 130 p. 51 - 59
Augmentative release of parasitoids against Ceratitis capitata (Weidemann) is currently at an early stage in Argentina. The success of this environment-friendly strategy depends on suitable mass-rearing procedures. The integration of radiation in the rearing process improves both mass production and handling of fruit ﬂy parasitoids. Diachasmimorpha longicaudata (Ashmead) is reared on larvae of tsl Vienna-8 C. capitata strain at the BioPlanta San Juan, Argentina. The aim of the current study was to identify the best combination of larval host age,exposuretime,andhostgamma-radiation dosetoachievethegreatestparasitoid massyieldwiththehighest female-biased oﬀspring ratio. The eﬀect of host radiation doses on parasitoid ﬁtness-related parameters from the ﬁrst ﬁlial generation was alsoassessed. Firstly, host larvae aged5?7d-old were exposed toparasitoids for 30, 60, 90, 120, 150 and 180min at a constant 4:1 host/parasitoid ratio. The most productive exposure procedure was 5?6d-old larvae for 1.30h. Secondly, batches of 180,000 naked host larvae aged 5?6d-old were irradiated at 0, 60, 65, 70, 75, 80, 85, 90, 95, and 100Gy. Suitable radiation doses for wholly suppressing host emergence wereat 85?100Gy. Thirdly, 5?6d-old host larvae irradiated at 85, 90, 95, and 100Gy were exposed to parasitoids. 6d-old larva irradiated at 90Gy was the most suitable combination to achieve the highest parasitoid emergence and female oﬀspring values. Increasing radiation doses beyond 90Gy did not signiﬁcantly enhance parasitoid yield and did not improve female-biased oﬀspring ratio. Fourthly, survival, fecundity, sex ratio, development time and parasitoid oﬀspring size were not adversely aﬀected by radiation. Gross fecundity and net reproductive rates, and sex ratio remarkably improved in comparison to that recorded on parasitoids reared on non-irradiated larvae. These ﬁndings greatly encourage the use of augmentative biological control against medﬂy in Argentina.