Somatic embryogenesis and plant regeneration through leaf culture in Arachis glabrata (Leguminosae)

MARÍA LAURA VIDOZ; HEBE YOLANDA REY; ANA MARÍA GONZALEZ; LUIS AMADO MROGINSKI

ACTA PHYSIOLOGIAE PLANTARUM

SPRINGER HEIDELBERG

Año: 2004 vol. 26 p. 59 - 66

0137-5881

Plants of two accessions of Arachis glabrata were regenerated via somatic embryogenesis. Embryogenic calli were initiated from leaflet explants on Murashige and Skoog  medium supplemented with picloram alone or picloram in combination with 6-benzylaminopurine. Leaflets of accession A6138 induced the highest percentage of  somatic embryos in media composed of 10 mg·dm-3 and 15 mg·dm-3 picloram. In contrast, 5 mg·dm-3 picloram with 0.1 mg·dm-3 6-benzylaminopurine was one of the most effective combinations in accession AF385. MS medium supplemented with 2 g·dm-3 activated charcoal (AC) used for 30 days was the most effective for embryo maturation. After 20 days of culture on MS medium devoid of growth regulators, 6 % of embryos converted into plantlets in accession A6138.