PERSONAL DE APOYO
LOPEZ APPIOLAZA Carlos Martin
artículos
Título:
HEAT SHOCK RESPONSE IN OXIDATIVE STRESS DURING NEONATAL UNILATERAL URETERAL OBSTRUCTION
Autor/es:
GARCÍA M, MAZZEI L, BENARDÓN ME, CACCIAMANI V, APPIOLAZA CM, RINALDI TOSSI M, BOCANEGRA V, VALLÉS P, MANUCHA W.
Revista:
BIOCELL
Editorial:
INST HISTOL EMBRIOL-CONICET
Referencias:
Lugar: Mendoza; Año: 2010 vol. 34 p. 46 - 46
ISSN:
0327-9545
Resumen:
Oxidative stress represents the common denominator of multiple
cellular alterations and contributes to tubulointerstitial mechanism
damage in neonatal unilateral ureteral obstruction (UUO).
Proposed alternatives of protection to renal injury include statin,
due to its effects decreasing oxidative stress and tubulointerstitial
fibrosis. Recently, we have demonstrated an association between
nitric oxide (NO) bioavailability and Hsp70 expression in UUO.
The goal was to evaluate possible antioxidant/antiapoptotic effects
of rosuvastatin (Ros) during UUO in order to establish its
nephroprotective effects. Neonatal rats (n=5) with and without UUO
and Ros treated rats (10 mg/Kg/d) for 14 days, were nephrectomized
to evaluate in cortexes oxidative stress and heat shock response.
After 14 days of obstruction, oxidative stress markers as
lipid peroxidation (MDA) (90±5 vs 70±4 nmol/mL) and NADPH
oxidase activity (21682±234 vs. 8200±123 RUF/μg prot/min) increased,
whereas hsf1 and Hsp70 expression (0.35±0.04 vs.
0.87±0.05) and lower endogenous nitric oxide levels (67±2 vs. 74±2
nM) decreased. Conversely, Rosuvastatin administration increased
hsf1 and Hsp70 expression linked to increase in NO levels with
absence of apoptotic response and decreased oxidative stress. These
findings suggest that Rosuvastatin exerts citoprotective effects
against oxidative stress through NO restoration and upregulation
of hsf1 and Hsp70 in neonatal unilateral ureteral obstruction.