Endocrine disruption in patagonian trout exposed to sewage effluents

GUERREÑO, MARIANA; LUQUET, CARLOS M; LO NOSTRO, FABIANA; CASTIÑEIRA LEANDRO; MAGGESE, MARÍA CRISTINA; GENOVESE, GRISELDA

New Orleans, LA, USA

Congreso; SETAC North America annual meeting; 2009

SETAC

<!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman","serif"; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:ES; mso-fareast-language:ES;} h2 {mso-style-unhide:no; mso-style-qformat:yes; mso-style-link:"Título 2 Car"; mso-style-next:Normal; margin-top:7.5pt; margin-right:-14.45pt; margin-bottom:7.5pt; margin-left:0cm; text-align:justify; line-height:150%; mso-pagination:widow-orphan; page-break-after:avoid; mso-outline-level:2; font-size:14.0pt; mso-bidi-font-size:12.0pt; font-family:"Times New Roman","serif"; mso-ansi-language:EN-US; mso-fareast-language:ES; font-weight:normal;} span.Ttulo2Car {mso-style-name:"Título 2 Car"; mso-style-unhide:no; mso-style-locked:yes; mso-style-link:"Título 2"; mso-ansi-font-size:14.0pt; mso-bidi-font-size:12.0pt; mso-ansi-language:EN-US; mso-fareast-language:ES;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt;} @page Section1 {size:612.0pt 792.0pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:36.0pt; mso-footer-margin:36.0pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> Endocrine disruption in patagonian trout exposed to sewage effluents.  1Guerreño, Mariana; 2,3Luquet, Carlos M; 1,2Lo Nostro, Fabiana; 4Leandro Castiñeira; 1,2Maggese, María Cristina; 1,2Genovese, Griselda  1Laboratorio de Embriología Animal, Departamento de Biodiversidad y Biología Experimental, Facultad de Cs. Exactas y Naturales, Universidad de Buenos Aires. C. Universitaria (C1428EHA), Buenos Aires, Argentina. 2CONICET. Rivadavia 1917 (C1033AAJ), Buenos Aires, Argentina. 3 Laboratorio de Ecotoxicología Acuática, INIBIOMA CONICET-UNCo Pasaje de la Paz 235, San Martín de los Andes 8370, Neuquén, Argentina. 4Estación de Captura y Reproducción de Salmónidos San Martín de los Andes. Dirección de Biología Acuática de la Provincia del Neuquén, Argentina.   e-mail: genovese@bg.fcen.uba.ar   <!-- /* Font Definitions */ @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-1610611985 1107304683 0 0 159 0;} @font-face {font-family:Calibri; panose-1:2 15 5 2 2 2 4 3 2 4; mso-font-charset:0; mso-generic-font-family:swiss; mso-font-pitch:variable; mso-font-signature:-1610611985 1073750139 0 0 159 0;} /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin-top:0cm; margin-right:0cm; margin-bottom:10.0pt; margin-left:0cm; line-height:115%; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-fareast-language:EN-US;} .MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:Calibri; mso-fareast-theme-font:minor-latin; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi; mso-fareast-language:EN-US;} .MsoPapDefault {mso-style-type:export-only; margin-bottom:10.0pt; line-height:115%;} @page Section1 {size:595.3pt 841.9pt; margin:70.85pt 3.0cm 70.85pt 3.0cm; mso-header-margin:35.4pt; mso-footer-margin:35.4pt; mso-paper-source:0;} div.Section1 {page:Section1;} --> The Lake Lacar, of glacial origin, ends in a relatively shallow bay of San Martín de los Andes (Patagonia, Argentina), a city which has demographically increased in the last few years. The Pocahullo River, the main affluent of the lake, crosses this city receiving both untreated domiciliary effluents and the discharge of the main sewage treatment plant of the city. Sewage effluents normally cause endocrine disruption in wild animals, evidenced by biomarkers such as the induction of female proteins, sex reversal, and reproductive impairment. We compared the effect of acute and chronic exposure of adult male Oncorhynchus mykiss (Teleostei, Salmoniformes) to Pocahullo River with control fish from unpolluted upstream areas (Centro de Ecología Aplicada del Neuquén). Surface mucus, blood, and organ samples were obtained for Western blots and histological analysis, respectively. Liver and gonad sections were stained with haematoxylin-eosin and further analysed under light microscopy. Marked alterations in the morphology of both organs were only detected in exposed male fish. These alterations included liver enlargement, euchromatic and basophilic hepatocytes, and abnormal spermatogenesis. We detected vitellogenin immunoreactive bands between 90-140 KDa in plasma from male fish submitted to chronic exposure. Such bands could not be detected in controls and fish exposed during 10 days to Pocahullo River, though hepatosomatic and gonadosomatic indices in exposed fish were augmented and reduced, respectively. This is the first report of xenoestrogenicity in a Patagonian fresh water salmonid fish. This work was supported by UBACyT X620, PICT2005 34417 and, CONICET PIP 6244.