INVESTIGADORES
LUQUET carlos Marcelo
congresos y reuniones científicas
Título:
Effects of azinphos-methyl on cellular immune responses of the freshwater snail Chilina gibbosa.
Autor/es:
CASTRO J.M.; BIANCHI, V.; HERBERT, L.T.; KRISTOFF, G.; LUQUET, C.M.
Lugar:
CABA
Reunión:
Congreso; SETAC Latin America 11th Biennial Meeting; 2015
Institución organizadora:
SETAC Latin America
Resumen:
Agriculture is one of the main economical activities in Upper Valley of Río Negro and Río Neuquén in Argentina. The use of pesticides released during these activities may negatively affect physiological responses of non-target exposed organisms. A recent study has shown cholinesterase activity inhibition and neurotoxic effects upon azinphos-methyl (AZM) exposure in Chilina gibbosa. Nevertheless, possible effects of this pesticide on the immune response have not been studied yet in this species. The aim of this work is to characterize the cellular immune response of C. gibbosa upon short-term exposure (48 h) to environmentally relevant concentrations of AZM. To achieve this, snails were collected from a site with no agrochemical pollution, and acclimated in laboratory. The following treatments were set without aeration or feeding: 1) Control, 2) Solvent control (acetone) and 3) AZM 20 μg/L, (n = 6, each one corresponding to pooled samples of hemolymph extracted from 3 snail independently treated). Data were compared by one-way ANOVA and post hoc comparisons. Results: Total hemocyte number showed no variation between treatments. Cellular viability significantly decreased upon solvent exposure, but this effect was greater when snails were exposed to AZM. Relative proportions of hyalocytes and round cells showed no variation between treatments; however, round cells were significantly more abundant than hyalocytes, in general. Granulocytes were not observed in any sample. Phagocytosis was mainly carried out by hyalocytes and was not affected by exposure to the solvent, but was significantly inhibited by AZM, related to the control. Conclusion: Short-term exposure to AZM negatively affects cell viability and phagocytic activity in hemocytes of C. gibbosa, producing no changes in the cellular type proportion.