INVESTIGADORES
LUQUET Carlos Marcelo
artículos
Título:
Acute neurotoxicity evaluation of two anticholinesterasic insecticides, independently and in mixtures, and a neonicotinoid on a freshwater gastropod
Autor/es:
HERBERT, LUCILA THOMSETT; COSSI, PAULA FANNY; PAINEFILÚ, JULIO CÉSAR; MENGONI GOÑALONS, CAROLINA; LUQUET, CARLOS MARCELO; KRISTOFF, GISELA
Revista:
CHEMOSPHERE
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2020
ISSN:
0045-6535
Resumen:
Neurotoxic insecticides are ubiquitous in aquatic ecosystems, frequently as part of complex mixtures.Freshwater gastropods are generally underrepresented in neurotoxicity evaluations and cumulativetoxicity testing. This study investigates the behavioural and biochemical effects of acute exposures to the carbamate carbaryl, the organophosphate chlorpyrifos, and the neonicotinoid acetamiprid on the freshwater gastropod Chilina gibbosa. First, we evaluated behavioural neurotoxicity and cholinesterase (ChE), carboxylesterase (CE), and glutathione S-transferase (GST) activities in acute (48h) single-chemical exposures to increasing concentrations of carbaryl (0.5e500 mg L1), chlorpyrifos (10e7500 mg L1 ), and acetamiprid (1e10000 mg L1). We then studied the effects of acute (48h) exposures to binary mixtures of carbaryl and chlorpyrifos equivalent to 0.5, 1, and 1.5 ChE 48h-IC50. None of the insecticides caused severe behavioural neurotoxicity, except for a significant lack of adherence by 5000 mg L1 chlorpyrifos. Carbaryl caused concentration-dependent inhibition of ChEs (NOEC 5 mg L1) and CEs with p-nitrophenyl butyrate as substrate (NOEC 5 mg L1; 48h-IC50 37 mg L1 ). Chlorpyrifos caused concentration-dependent inhibition of ChEs (NOEC 50 mg L1; 48h-IC50 946 mg L1) but did not affect CEs (NOEC 7500 mg L1). Carbaryl-chlorpyrifos mixtures inhibited ChEs additively, inhibited CEs with p-nitrophenyl butyrate, and did not affect behaviour. GST activity was not affected by single or mixture exposures. Acute exposure to acetamiprid did not affect any of the endpoints evaluated. This study provides new information on carbaryl, chlorpyrifos, and acetamiprid toxicity on C. gibbosa, relevant to improve gastropod representation in ecotoxicological risk assessment