INVESTIGADORES
LUQUET Carlos Marcelo
artículos
Título:
Transepithelial potential differences and Na+ flux in isolated perfused gills of the crab Chasmagnathus granulatus (Grapsidae) acclimated to hyper- and hypo-salinity.
Autor/es:
14. LUQUET C; POSTEL U; URCOLA M; HALPERIN J; MARQUES R; SIEBERS D.
Revista:
JOURNAL OF EXPERIMENTAL BIOLOGY
Editorial:
The Company of Biologists
Referencias:
Lugar: Cambridge; Año: 2002 vol. 205 p. 71 - 77
ISSN:
0022-0949
Resumen:
We studied the transepithelial potential difference (TEPD) and 22Na flux across isolated perfused gills (anterior pair 5 and posterior pairs 6–8) of the crab22Na flux across isolated perfused gills (anterior pair 5 and posterior pairs 6–8) of the crab Chasmagnathus granulatus acclimated to either hypo- or hyper-osmotic conditions. The gills of crabs acclimated to low salinity, perfused and bathed with 10 ‰ saline solutions, produced the following TEPDs (hemolymph side with respect to bath side): 0.4±0.7, –10.2±1.6, –10.8±1.3 and –6.7±1.3 mV for gills 5, 6, 7 and 8, respectively. Gills 6, 7 and 8 did not differ significantly. Reducing the saline concentration of bath and perfusate from 30 ‰ to 20 ‰ or 10 ‰ increased significantly the TEPDs of these gills. TEPDs of gill 6 (representative of posterior gills) were reduced by 69±5% and 60±5% after perfusion with ouabain or BaCl2acclimated to either hypo- or hyper-osmotic conditions. The gills of crabs acclimated to low salinity, perfused and bathed with 10 ‰ saline solutions, produced the following TEPDs (hemolymph side with respect to bath side): 0.4±0.7, –10.2±1.6, –10.8±1.3 and –6.7±1.3 mV for gills 5, 6, 7 and 8, respectively. Gills 6, 7 and 8 did not differ significantly. Reducing the saline concentration of bath and perfusate from 30 ‰ to 20 ‰ or 10 ‰ increased significantly the TEPDs of these gills. TEPDs of gill 6 (representative of posterior gills) were reduced by 69±5% and 60±5% after perfusion with ouabain or BaCl22 (5mmoll–1 each), respectively. The same gill showed a net ouabain-sensitive Na+ influx of 1150±290 mequiv g–1 h–1. Gill 6 of crabs acclimated to high salinity produced TEPDs of –1.5±0.1 and –1.3±0.09 mV after perfusion with 30 ‰ or 40 ‰ salines, respectively. Perfusion with ouabain or BaCl2 reduced TEPDs by 76±7% and 86±4%, respectively. A net ouabain-sensitive Na+ efflux of 2282±337 mequiv g–1h–1 was recorded in gill 6 perfused with 38 ‰ saline.2 reduced TEPDs by 76±7% and 86±4%, respectively. A net ouabain-sensitive Na+ efflux of 2282±337 mequiv g–1h–1 was recorded in gill 6 perfused with 38 ‰ saline.+ efflux of 2282±337 mequiv g–1h–1 was recorded in gill 6 perfused with 38 ‰ saline.–1 each), respectively. The same gill showed a net ouabain-sensitive Na+ influx of 1150±290 mequiv g–1 h–1. Gill 6 of crabs acclimated to high salinity produced TEPDs of –1.5±0.1 and –1.3±0.09 mV after perfusion with 30 ‰ or 40 ‰ salines, respectively. Perfusion with ouabain or BaCl2 reduced TEPDs by 76±7% and 86±4%, respectively. A net ouabain-sensitive Na+ efflux of 2282±337 mequiv g–1h–1 was recorded in gill 6 perfused with 38 ‰ saline.2 reduced TEPDs by 76±7% and 86±4%, respectively. A net ouabain-sensitive Na+ efflux of 2282±337 mequiv g–1h–1 was recorded in gill 6 perfused with 38 ‰ saline.+ efflux of 2282±337 mequiv g–1h–1 was recorded in gill 6 perfused with 38 ‰ saline.+ influx of 1150±290 mequiv g–1 h–1. Gill 6 of crabs acclimated to high salinity produced TEPDs of –1.5±0.1 and –1.3±0.09 mV after perfusion with 30 ‰ or 40 ‰ salines, respectively. Perfusion with ouabain or BaCl2 reduced TEPDs by 76±7% and 86±4%, respectively. A net ouabain-sensitive Na+ efflux of 2282±337 mequiv g–1h–1 was recorded in gill 6 perfused with 38 ‰ saline.2 reduced TEPDs by 76±7% and 86±4%, respectively. A net ouabain-sensitive Na+ efflux of 2282±337 mequiv g–1h–1 was recorded in gill 6 perfused with 38 ‰ saline.+ efflux of 2282±337 mequiv g–1h–1 was recorded in gill 6 perfused with 38 ‰ saline.mequiv g–1h–1 was recorded in gill 6 perfused with 38 ‰ saline.