INVESTIGADORES
PENA liliana Beatriz
congresos y reuniones científicas
Título:
Copper stress induces hormonal changes and protein modifications in the roots of maize seedlings
Autor/es:
CL MATAYOSHI; LB PENA; MS ZAWOZNIK; V ARBONA; A GÓMEZ-CADENAS; SM GALLEGO
Lugar:
Paraná
Reunión:
Congreso; LIV Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2018
Institución organizadora:
SAIB
Resumen:
The excess of Cu interferes with numerous biological functions that can affect plant growth. In the current study we present the impact of Cu stress on root of Zea mays L. (maize) seedlings regarding growth and hormone homeostasis. Maize seeds were germinated on filter paper in plastic dishes containing distillate water. Seedlings of a comparable size with roots of 1-2 cm length were selected and transferred to hydroponic culture containing diluted (1/10) Hoagland solution without (control, C) or with 50 or 100 μM of CuCl2. Plants were grown in a controlled climate room at temperature of 24 ± 2 °C in darkness and, after 72 h of treatment, determinations were performed. The root apex (5 mm) segment and the rest of the root tissue were processed separately. The decrease in root length and biomass by Cu were accompanied by drastic reductions of absicic acid, indoleacetic acid, jasmonic acid (JA), isoleucine conjugate of JA, and giberellins GA4 and GA3 levels in the root apex. This was accompanied by an increase in protein carbonylation, ubiquitylation and SUMOylation in both parts of the root. Our results show that root hormonal imbalance produced by Cu exposure is -at least in part- responsible of the root growth inhibition. Also, severe protein post-translational modifications upon Cu-exposure occurred that took place in the entire root and are probably important to trigger and/or to sustain defence mechanisms against heavy metal toxicity.