Relationship between molecular and enological features of patagonian wine yeasts: relevance in selection protocols

LOPES C. A.; RODRÍGUEZ M. E.; QUEROL A.; BRAMARDI S.; CABALLERO A. C.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Springer

Año: 2006 vol. 22 p. 827 - 833

0959-3993

In this paper, a relation study between genetic patterns, obtained by the combination of mtDNA-RFLP and PCR amplified inter d sequence DNA polymorphism analysis, and enological relevant phenotypic data (fermentative power, specific productivity, volatile and total acidity) was carried out from Argentinean north-Patagonian Saccharomyces cerevisiae isolates. The use of a powerful statistical tool as the Generalized Procrustes Analysis allow us to weigh the relationship for each isolate in particular, denoting a good enough degree of agreement between molecular and physiological data for most of the population analyzed. The inclusion of a physiological feature, as the killer sensitivity biotype, within identification methods resulted in a higher degree of discrimination among isolates and in better correlation between both characterizations. The combined use of methods based on molecular polymorphisms and killer biotype could be applied so as not to miss any isolate with differential enological properties in selection protocols.