INVESTIGADORES
KOLMAN Maria De Los Angeles
congresos y reuniones científicas
Título:
SOIL MICROALGAE FROM MISIONES PROVINCE WITH PLANT GROWTH STIMULANT POTENTIAL
Autor/es:
MIÑO, ML; ZAPATA, PD; KOLMAN, MA
Lugar:
Los Cocos, Cordoba
Reunión:
Congreso; XVII Congreso Argentino de Microbiología; 2022
Institución organizadora:
Sociedad Argentina de Microbiologia
Resumen:
Microalgae are oxygenic photosynthetic microorganisms that inhabit a wide variety of environments. In recent years, interest in the use of microalgae for biotechnological exploitation has increased, being one of the main advantages the low nutritional requirements for their cultivation. Its use in modern agriculture is currently being explored because, in addition to improving fertility and soil quality, microalgae can also produce plant growth hormones, polysaccharides, antimicrobial compounds and other metabolites to promote plant growth.The objective of this study was to collect, isolate and identify soil microalgae from Misiones and study their effects as a biostimulant of plant growth. Soil samples were obtained from Santo Pipó and Comandante Andresito, Misiones and cultured in BG11 media. By isolation in solid BG11 media, 12 axenic cultures were obtained. For molecular identification, genomic DNA was extracted and PCR amplification was performed using ITS1-5.8S-ITS2 primers. Amplicons were sequenced and analysed using the BLAST tool of the NCBI . Multiple sequence alignment was carried out using the ClustalW and phylogenetic trees were constructed using the MEGA software. We identified 9 microalgae genres present in soil: Eustigmatos, Graesiella, Chlamydomonas and Chlorococcum. Graesiella emersonii and Chlorococcum vacuolatum were selected to test the biostimulant effect on tomato plants. The strains were grown in BG11 at 25°C with a light intensity of 50 μmol photons.m-2.s-1 with a 14:10 light: dark cycle until the exponential phase (OD750=1.25). Then, sterile soil was inoculated with the algae and kept in the same conditions for 10 days. Finally, this inoculum was mixed with sterile soil in a 1:8 (p/p) ratio. Seedlings were kept at 25°C with a light intensity of 50 μmol photons.m-2.s-1 for 14:10 light: dark cycle irrigated every 2 days and harvested 20 days after the appearance of the first pair of true leaves. Results showed that G. emersonii significantly increased the height, length and root dry weight of tomato plants. While C. vacuolatum only had a significant effect on seedling height. These results allow us to conclude that these isolated soil strains have the potential to function as plant growth stimulants.