INVESTIGADORES
KOLMAN Maria De Los Angeles
congresos y reuniones científicas
Título:
Current status and perspectives of unificiation of Glu-3 nomenclature systems in common wheat.
Autor/es:
IKEDA, TM; BRANLARD, G; PEÑA RJ; TAKATA K; LIU L; HE Z; APPELBEE M; HURKMAN I; LERNER, SE; KOLMAN, MA; ROGERS, WJ
Reunión:
Conferencia; 8th International Wheat Conference; 2010
Resumen:
Low-molecular-weight glutenin subunit (LMW-GS) composition in common wheat is one of the critical determinants of gluten properties. However, the nomenclature of Glu-3 encoding LMW-GSs has not been consistent among laboratories, due to the complexity of the LMW-GSs and the distinct separation methods used by different researchers. It is very important to unify the nomenclature systems in current use, to facilitate the sharing of information about the effects of individual LMW-GS on gluten properties. We therefore shared cultivars having various Glu-A3, Glu-B3 and Glu-D3 alleles from various countries. Using 1D SDS-PAGE, 2D and MS analyses, we found differences in nomenclature particularly for Glu-A3 and Glu-B3, including new Glu-3 alleles. For 1D SDS-PAGE, various running condition were compared to identify these alleles efficiently. We propose a new list of standard cultivars representing Glu-3 alleles and standard SDS-PAGE methods to identify them.