INVESTIGADORES
KOLMAN Maria De Los Angeles
congresos y reuniones científicas
Título:
Occurrence and phylogenetic studies of the microcystin biosynthesis genes
Autor/es:
SAKAMOTO, T; RODRIGUES DE MELO, V; KOLMAN, MA; MUNIZ GUEDES, RL; GIANI, A; ORTEGA, JM
Lugar:
Sao Paulo
Reunión:
Conferencia; 8th International Conference of the Brazilian Association for Bioinformatics and Computational Biology; 2012
Institución organizadora:
Universidade Federal de Campinas
Resumen:
Non-ribosomal peptide synthesis machinery is largely used by cyanobacteria for synthesis of several secondary metabolites, including microcystin. The main complex involved in this process is NRPS (non-ribosomal peptide synthetase) which has modular structure containing, each module, an AMP-binding domain (AMPb-domain) and a condensation domain (Cond-domain). Each module is responsible for incorporation of one specific amino acid to the product and is repeated through NRPS structure. In this study, we performed an evolutionary study using phylogenetic approach of this complex having the microcystin biosynthesis genes as a model. Microcystin is specifically synthesized in some cyanobacteria like Microcystis, Anabaena and Planktothrix through a set of about 10 genes organized in operons. Among them, 5 genes (mcyA, mcyB, mcyC, mcyE and mcyG) contain the modular structure of NRPS.