International collaboration for unifying Glu-3 nomenclature systems in common wheat

IKEDA, TM; BRANLARD, G; PEÑA RJ; TAKATA K; LIU L; HE Z; LERNER, SE; KOLMAN, MA; YOSHIDA Y; ROGERS, WJ

Proceedings 11th International Wheat Genetics Symposium.

Sydney University Press

Lugar: Sydney; Año: 2008; p. 130 - 133

Low-molecular-weight glutenin subunit (LMW-GS) composition in common wheat is one of the critical determinants of gluten properties. However, the nomenclature of Glu-3 encoding LMW-GSs has not been consistent among laboratories, due to the complexity of the LMW-GSs and the distinct separation methods used by different researchers. It is very important to unify the nomenclature systems in current use, to facilitate the sharing of information about the effects of individual LMW-GS on gluten properties. We therefore shared 103 cultivars having various Glu-A3, Glu-B3 and Glu-D3 alleles from Argentina, China, France, Japan and Mexico. Using 1D SDS-PAGE and 2D analyses, we found differences in nomenclature particularly for Glu-A3 and Glu-B3, including new Glu- 3 alleles among laboratories. We propose a new list of standard cultivars representing Glu-3 alleles.