ROGERS William john
congresos y reuniones científicas
Update on low-molecular-weight glutenin subunit identification
IKEDA, T.M.; ROGERS, W.J.; BRANLARD, G.; PEÑA, R.J.; LERNER, S.E.; ARRIGONI, A.; MA, W.J.; APPELS, R.; LUKOW, O.; HURKMAN, W.; APPELBEE, M.; SISSONS, M.; CARRILLO, J.M.; HE, Z.H.
Congreso; 11th International Gluten Workshop; 2012
Allelic variation for the low-molecular-weight glutenin subunits (LMW-GS) is a major determinant of differences in dough viscoelastic properties observed between cultivars of both bread wheat and durum. Technical difficulties in allelic identification due to the complexity of the protein profile produced by each cultivar and the use of different nomenclature systems in different laboratories has historically interfered with information exchange between research groups, a situation exacerbated by the vast number of possible profiles found in different cultivars due to the multi-allelic nature of the principal loci encoding LMW-GSs (Glu-A3, Glu-B3 and Glu-D3). These various difficulties prompted research workers at CAAS, CIMMYT, INRA, NARO and UNCPBA to form an international collaborative group aimed at unifying criteria across laboratories and comparing four different methods of allelic identification (SDS-PAGE, 2-DE, MALDI-TOF-MS and PCR). The current contribution summarises progress to date made by this group in studies on bread wheat, seeks to address remaining challenges and places the findings in the context of the wheat gene catalog. We also propose the formation of a wider international group aimed at facilitating the resolution of the remaining problems in the field.