GONZALEZ Ana Maria
Somatic embryogenesis and plant regeneration from immature zygotic embryos of Melia azedarach (Meliaceae).
S. VILA; GONZALEZ, ANA MARIA; H. REY; L. MROGINSKI
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY. PLANT
Año: 2003 vol. 39 p. 283 - 287
A procedure for the regeneration of paradise tree (Melia azedarach, Meliaceae ) plants from immature zygotic embryos via somatic embryogenesis was developed. Somatic embryos were induced from explants cultured on Murashige and Skoog medium supplemented with 0.45, 4.54, or 13.62mM thidiazuron. Histological examination revealed that somatic embryos were induced directly from the explants. Further development of somatic embryos was accomplished with Murashige and Skoog medium at quarter-strength with 3% sucrose. A large number of plants were regenerated from somatic embryos and successfully established in soil in a greenhouse. These plants are morphologically similar to those of seed-derived plants. This system may be beneficial for mass propagation as well as for genetic manipulation of the paradise tree.