INVESTIGADORES
GONZALEZ Ana Maria
artículos
Título:
Plant regeneration in Arachis pintoi (Legunminosae) through live culture
Autor/es:
H.Y. REY; A. SCOCCHI,; GONZALEZ, ANA MARIA; L. MROGINSKI
Revista:
PLANT CELL REPORTS
Editorial:
Springer Verlag Ed
Referencias:
Año: 2000 vol. 19 p. 856 - 862
ISSN:
0721-7714
Resumen:
Abstract:  Abstract Plant regeneration in Arachis pintoi was obtained via two developmental pathways: organogenesis and somatic embryogenesis. Organogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with NAA or 2,4-D in combination with BA, KIN or 2iP. The most suitable combination for plant regeneration through organogenesis was an initial medium composed of 10 mg/l NAA+1 mg/l BA followed by transfer of the callus to a shoot induction medium (MS+1 mg/l BA). Rooting of regenerated shoots was readily achieved by culture on MS+0.01 mg/l NAA. Embryogenic callus cultures were initiated from pieces of leaf on MS medium supplemented with PICL in combination with KIN, ZEA, BA or 2iP, and the most suitable combinations were 20 mg/l PICL+1 mg/l BA or 2iP. When pieces of embryogenic callus were subcultured on MS+1 mg/l BA, somatic embryos were differentiated and developed further into well-developed plants in MS+1 g/l AC followed by MS medium devoid of plant growth regulators.