Mark-Houwink parameters of Biosynthetic Poly(g-Glutamic Acid in Aqueous Solution.

I. M. IRURZUN; J. J. BOU; G. PÉREZ-CAMERO; C. ABAD; A. CAMPOS; S. MUÑOZ GERRA

MACROMOLECULAR CHEMISTRY AND PHYSICS

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2001 vol. 202 p. 3253 - 3256

1022-1352

A combined viscosity-light scattering-gel permeation chromatography (GPC) study was carried out on bacterially produced poly( gamma-glutamic acid) (PGGA). PGGA samples with weight-average molecular weights ranging from 8×104 up to 8×105 g·mol-1 dissolved in phosphate buffer at 0.13 M ionic strength were used. It was found that the Mark-Houwink relation is acceptably obeyed, giving K and a values of 1.84×10-6 dL·g-1 and 1.16, respectively. As expected, GPC analysis showed that PGGA does not follow the universal calibration plot and that deviations can not be avoided by modifying the ionic strength.