Application Of The ERIC-PCR Technique As A Tool For Following DNA Damage During The Water Disinfection Process With UVC

CRISTIANI MARIANA; MARINA FLORES; BRANDI RODOLFO; LABAS MARISOL

Natal, Rio Grande del Norte

Conferencia; 4th Iberoamerican Conference on Advanced Oxidation Technologies; 2019

Universidad Federal de Rio Grande del Norte (UFRN)- Natal,

An interesting and novel concept to apply in the follow-up of the water disinfection process is to do it through the damage produced by the disinfectant agent on sensitive components of microorganisms. In this work the ERIC-PCR technique to monitor DNA damage was used. The experimental development was carried out in a batch reactor, using three intensities of UV-C radiation (10.5, 4.2 and 2.1 mW/cm2). For each sample, a DNA extraction was performed for the evaluation by ERIC-PCR. Semi-quantitative information was obtained on the inactivation process of the agarose gels by densitometries that determined the value of the areas of each DNA fragment generated. A linear correlation was obtained with a high correspondence, between the inactivation index obtained through the microbial counts (INACI) and the DNA damage index (DNADI) that was expressed as DNADI = 0.05726 × INACI. It was concluded that the ERIC PCR is a feasible and adequate tool for the detection, estimation and monitoring of DNA damage in the processes of disinfection of water with UV radiation.